HigherPurity™ FFPE DNA Isolation Kit
HigherPurity™ FFPE DNA Isolation Kit provides a fast, safe, and xylene-free method for extracting high-quality DNA from formalin-fixed, paraffin-embedded (FFPE) tissues.
Its optimized two-phase protocol (Deparaffinization + DNA Extraction) ensures pure, inhibitor-free DNA, ideal for PCR, qPCR, NGS, and STR analysis, even from long-archived samples.
HigherPurity™ FFPE DNA Isolation Kit provides an easy, rapid, and highly efficient method for extracting and purifying DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples, the most common form of long-term preservation for archived pathological specimens.
The extraction process consists of two optimized phases:
1) Deparaffinization, using a proprietary non-toxic buffer that completely dissolves paraffin without the use of flammable or malodorous solvents such as xylene or d-limonene.
2) DNA Isolation, delivering high-quality, inhibitor-free DNA ideal for sensitive downstream molecular applications.
The result is high-purity DNA suitable for reliable PCR, qPCR, NGS, or STR analysis, even from decades-old FFPE samples.
Have questions? Contact us
Price: Price range: 216.00 € through 844.80 €
Detailed information:
Advantages & Features
- High-quality DNA: suitable for all molecular biology downstream applications.
- Fast and simple: total extraction process completed in less than 2 hours 45 min with minimal hands-on time.
- Safe and solvent-free: eliminates the need for xylene or other toxic organic solvents.
- High purity and yield: complete removal of contaminants and PCR inhibitors.
- Low elution volume: concentrated DNA recovery between 15–30 µL.
- Long-term preservation: optimized for stable extraction from archived FFPE samples.
- Comprehensive kit: includes all reagents for both Deparaffinization and DNA Extraction phases.
Specifications
Includes
Includes for 50 rxn:
– 30 mL Deparaffinization solution
– 10 mL Tissue Lysis Buffer
– 15 mL Lysis/ Binding Buffer
– 2 x 30 mg Proteinase K
– 18 mL Desinhibition Buffer
– 10 mL Wash Buffer
– 10 mL Elution Buffer
– 50 units MicroSpin Columns
– 100 units Collection tubes
Download documentation
Datasheet MSDSApplications
- Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples.
- Isolation of DNA from fresh and archived FFPE samples.
- Isolation of DNA from specimen of object slides.
- Typical downstream applications such as:
- PCR & qPCR
- Next-Generation Sequencing
- Short Tandem Repeat (STR) analysis (RUO)
- Mutation screening and genotyping (RUO
Tables & Figures
t
Quality Control
- DNA isolated from tissue slice Paraffin-embedded (10 µm) quantified by Spectrophotometer and analysed by PCR.
Advice
Storage & Shipping
- Shipped in: Ambient Temperature.
- Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.
Citations
Sevilla, A., Morales, M. C., Ezkurra, P. A., Rasero, J., Velasco, V., Cancho-Galan, G., … & Asumendi, A. (2020). BRAF V600E mutational load as a prognosis biomarker in malignant melanoma. PLOS ONE, 15(3), e0230136.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
FFPE DNA extraction kit, DNA isolation from FFPE samples, Formalin-fixed paraffin-embedded DNA kit, FFPE genomic DNA purification kit, DNA extraction FFPE tissue
Also known as:
- Spanish: Kit de extracción de ADN FFPE, Aislamiento de ADN de muestras FFPE, ADN de tejido parafinado
- French: Kit d’extraction ADN FFPE, Isolation ADN échantillons FFPE, ADN tissus paraffinés
- German: FFPE-DNA-Extraktionskit, DNA-Isolation aus FFPE-Proben, DNA aus paraffinierten Geweben
- Italian: Kit estrazione DNA FFPE, Isolamento DNA campioni FFPE, DNA tessuti paraffinati
FAQs
2 reviews for HigherPurity™ FFPE DNA Isolation Kit
You may also like…
-
PRImeZOL™ Reagent
Price: 91.70 € AN1100Add to basket -
HigherPurity™ Bacterial DNA Isolation Kit
Price: Price range: 96.90 € through 313.90 € AN0066Select options This product has multiple variants. The options may be chosen on the product page -
HigherPurity™ Tissue DNA Extraction Kit (Reagent)
Price: Price range: 91.20 € through 423.00 € AN0052Select options This product has multiple variants. The options may be chosen on the product page -
HigherPurity™ Stool DNA Isolation Kit
Price: Price range: 155.30 € through 532.70 € AN0130Select options This product has multiple variants. The options may be chosen on the product page
Martin Vogel –
I use this kit when I need to recover DNA from paraffin-embedded tissue sections. Having a workflow specifically intended for both fresh and archived FFPE samples is useful for the type of material I handle. The isolated DNA can subsequently be used for PCR, qPCR or NGS, so I do not need a separate purification approach for these downstream steps.
Rachel Turner –
We introduced this kit for archived FFPE research material. The workflow is clearly organized, making it easier to process these types of samples alongside our routine extractions.