HigherPurity™ Tissue Total RNA Purification Kit

Includes for 50 rxn:
– 20 mL Buffer BLY
– 27 mL Wash Buffer 1
– 16 mL Wash Buffer 2
– 5 mL RNase-free ddH2O
– 50 RNAprep spin column
– 50 Filter Column
– 100 Collection tube (2 mL)
– 50 microtube (1.5 mL)
– 50 Micropestle

Purified RNA suitable for all common Molecular Biology applications, such as:

• RT-PCR.
• Northern blotting.
• cDNA library construction.
• Gene expression analysis.
• RNA quantification and integrity assessment.

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• Total RNA is isolated from a 30 mg thorax muscle tissue sample.
• Purified RNA is quantified using a spectrophotometer with a typical yield of more than 10 µg of Total RNA and an A260nm/A280nm ratio of 1.9-2.1.
• Quality is further checked by agarose gel electrophoresis.

• Shipped in: Ambient Temperature.
• Storage: Room Temperature (15–25 °C).

• Maestro, J. L., Maya, X., Rey-Alfonso, Á., & Escudero, J. (2026) Insulin Receptor-2 regulates 20-hydroxyecdysone synthesis and ecdysis in the German cockroach. Available at SSRN 6302826.
• Gonzalvo, J., Escudero, J., & Belles, X. (2026). Chinmo affects developmental timing and patterning during cockroach embryogenesis. PloS one, 21(5), e0347492.
• Escudero, J., Gonzalvo, J., Piulachs, M. D., & Belles, X. (2025). Chinmo function in cockroaches provides new insights into the regulation and evolution of insect metamorphosis. PLoS genetics, 21(12), e1011993.
• Wu, Y., Rashidpour, A., Duan, W., Fàbregas, A., Almajano, M. P., & Metón, I. (2025). Chitosan-Mediated Expression of Caenorhabditis elegans fat-1 and fat-2 in Sparus aurata: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors. Marine Drugs, 23(11), 434.
• Pagone, V., Pujal, D., & Maestro, J. L. (2025). An insulin-like peptide specific for a cockroach male reproductive gland. PloS one, 20(8), e0329852.
• Pujal, D., Escudero, J., Cabrera, P., Bos, L., Vargas-Chávez, C., Fernández, R., Bellés, X. & Maestro, J. L. (2024). Functional redundancy of the three insulin receptors of cockroaches. Insect Biochemistry and Molecular Biology, 104161.
• Balart-García, P., Aristide, L., Bradford, T., Beasley-Hall, P., Polak, S., Ribera, I., … & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
• Domínguez, C. V., Pagone, V., & Maestro, J. L. (2021). Regulation of insulin-like peptide expression in adult Blattella germanica females. Insect Biochemistry and Molecular Biology, 103706.
• Alvariño, R., Alonso, E., Alfonso, A., & Botana, L. M. (2019). Neuroprotective Effects of apple‐derived Drinks in a Mice Model of Inflammation. Molecular Nutrition & Food Research.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Tissue RNA extraction kit, Total RNA isolation from tissue, RNA purification kit tissue samples, Tissue RNA isolation kit, RNA extraction tissue kit

Also known as:

• Spanish: Kit de extracción de ARN de tejidos, Aislamiento de ARN total de tejidos, Purificación de ARN de tejidos
• French: Kit d’extraction ARN tissus, Isolation ARN total tissus, Purification ARN tissus
• German: RNA-Extraktionskit für Gewebe, Gesamt-RNA-Isolation aus Gewebe, RNA-Reinigung Gewebe
• Italian: Kit estrazione RNA tessuti, Isolamento RNA totale da tessuti, Purificazione RNA tessuti

Q: Can this kit be used with RT-qPCR workflows?
A: Yes, the extracted RNA is suitable for downstream applications such as real-time RT-PCR.

Q: What alternatives are available for tissue homogenization without using a mortar?
A: The kit includes micropestles and is compatible with mechanical homogenizers or liquid nitrogen grinding.

Q: How should complex tissues be handled during lysis?
A: Ensure efficient mechanical disruption, sufficient lysis buffer, and proper clarification to avoid column clogging.